Impact of different oral treatments on the composition of the supragingival plaque microbiome

• Published in: Journal of oral microbiology Vol. 14; no. 1; p. 2138251
• Main Authors: Rabe, Alexander, Gesell Salazar, Manuela, Michalik, Stephan, Kocher, Thomas, Below, Harald, Völker, Uwe, Welk, Alexander
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 31.12.2022; Taylor & Francis Group
• Subjects: dental plaque; essential oil; healthy human oral microbiome; lactoperoxidase; metaproteomics; nLC-MS/MS; Original; Teeth
• ISSN: 2000-2297; 2000-2297
• DOI: 10.1080/20002297.2022.2138251

Antiseptics are used to inhibit oral biofilm growth. However, they affect not only pathogenic but also commensal bacteria, which are a natural barrier against oral diseases. Using a metaproteome approach combined with a standard plaque-regrowth study, this pilot study examined the impact of different concentrations of lactoperoxidase (LPO)-system containing lozenges on early plaque formation, and active biological processes. Sixteen orally healthy subjects received four local treatments as a randomized single-blind study based on a cross-over design. Two lozenges containing components of the LPO-system in different concentrations were compared to a placebo and Listerine®. The newly formed dental plaque was analyzed by mass spectrometry (nLC-MS/MS). On average 1,916 metaproteins per sample were identified, which could be assigned to 116 genera and 1,316 protein functions. Listerine® reduced the number of metaprotein groups and their relative abundance, confirming the plaque inhibiting effect. The LPO-lozenges triggered mainly higher metaprotein abundances of early and secondary colonizers as well as bacteria associated with dental health but also periodontitis. Functional information indicated plaque biofilm growth. The effects of Listerine® and LPO-system containing lozenges used for plaque inhibition are different. In contrast to Listerine®, the lozenges allowed maintenance of a higher bacterial diversity.