Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

• Published in: Cytotechnology (Dordrecht) Vol. 23; no. 1-3; pp. 61 - 75
• Main Authors: MARTENS, D. E, NOLLEN, E. A. A, HARDEVELD, M, VAN DER VELDEN-DE GROOT, C. A. M, DE GOOIJER, C. D, BEUVERY, E. C, TRAMPER, J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1997; Springer Nature B.V; Kluwer Academic Publishers
• Subjects: Animal cells; Biological and medical sciences; Biotechnology; Cell death; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Miscellaneous; Vero cells; Cell culture; Airlift reactor; Shear; Hydrodynamic force; Mortality; Monkey; Kidney; Kinetic model; Bioreactor; Vertebrata; Cell line; Mammalia; Microcarrier cell culture; Animal; Primates; Macroporosity
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1023/B:CYTO.0000010399.23373.65

The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.