Improvement of catechin productivity in suspension cultures of tea callus cells

• Published in: Biotechnology progress Vol. 19; no. 2; pp. 655 - 658
• Main Authors: Shibasaki-Kitakawa, N, Takeishi, J, Yonemoto, T
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 2003; American Institute of Chemical Engineers
• Subjects: Biological and medical sciences; Bioreactors - microbiology; callus culture; Camellia sinensis; Camellia sinensis - cytology; Camellia sinensis - growth & development; Camellia sinensis - metabolism; Camellia sinensis - radiation effects; Catechin - biosynthesis; cell culture; Cell Culture Techniques - methods; Cell Division - radiation effects; Cells, Cultured; Culture Techniques - methods; Dose-Response Relationship, Radiation; flavonoids; Flavonoids - biosynthesis; Fundamental and applied biological sciences. Psychology; Light; Plant Leaves - cytology; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Leaves - radiation effects; Quality Control
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp025539a

In the suspension cultures of tea callus cells, C.sinensiscv. Yabukita, the effects of the culture conditions, such as culture period and light irradiation, on cell growth and catechin production were investigated. The production of flavonoids (catechins + proanthocyanidins) was promoted by inoculating the cells into the fresh medium at the culture period giving the maximum flavonoid content in the cells. The cultivation under light irradiation was repeated several times by inoculating the cells with the maximum flavonoid content. The flavonoid production was significantly increased without inhibiting the cell growth. We obtained the maximum flavonoid production, 1.5 g/dm3 medium, and the maximum content, 150 mg/(g of dry cell weight (DCW)). The latter value was larger than that in the leaves of the tea plant.