Isolation and characterization of microsatellite loci for the Asian longhorned beetle, Anoplophora glabripennis

We describe the first isolation of SSR (simple sequence repeat) markers for Anoplophora glabripennis, a pest of forests in China and urban trees in the USA. Ten markers were developed using FIASCO (fast isolation by AFLP of sequences containing repeats) with an (AC)₁₇ probe, and five markers were id...

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Published inMolecular ecology resources Vol. 9; no. 3; pp. 925 - 928
Main Authors CARTER, MAUREEN, CASA, ALEXANDRA M, ZEID, MAHMOUD, MITCHELL, SHARON E, KRESOVICH, STEPHEN
Format Journal Article
LanguageEnglish
Published Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.05.2009
Blackwell Publishing Ltd
Subjects
Anoplophora glabripennis
Asian longhorned beetle
Coleoptera
SSR
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Summary:We describe the first isolation of SSR (simple sequence repeat) markers for Anoplophora glabripennis, a pest of forests in China and urban trees in the USA. Ten markers were developed using FIASCO (fast isolation by AFLP of sequences containing repeats) with an (AC)₁₇ probe, and five markers were identified in expressed sequence tags found on the NCBI database. Twenty-seven beetles from a population in Gansu Province, China, were assayed. The number of alleles ranged from 3 to 9; observed and expected heterozygosities ranged from 0.07 to 0.79 and from 0.30 to 0.78, respectively.
Bibliography:http://dx.doi.org/10.1111/j.1755-0998.2008.02485.x
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ArticleID:MEN2485
istex:3EEB41A17E4625706775DE15322A94E37FED9FCF
doi: 10.1111/j.1755‐0998.2008.02485.x
Present address: Nature Source Genetics, 33 Thornwood Drive, Ithaca, NY 14850, USA.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1755-0998
1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2008.02485.x