Bioconversion of mango (Mangifera indica) seed kernel starch into bioethanol using various fermentation techniques

• Published in: Heliyon Vol. 8; no. 6; p. e09707
• Main Authors: Awodi, P.S., Ogbonna, J.C., Nwagu, T.N.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.06.2022; Elsevier
• Subjects: Bioethanol production; Mango; Mango seed kernel; Mango seed starch; Starch hydrolysis; Sugar fermentation; Bioethanol production; Mango; Sugar fermentation; Mango seed kernel; Mango seed starch; Starch hydrolysis
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2022.e09707

The potential of mango seed kernel starch (MKS) as a feedstock for bioethanol production was evaluated in this study. Starch extraction and hydrolysis from mango kernel were studied. Fermentation methods included separate hydrolysis and fermentation (SH&F), simultaneous saccharification and co-fermentation (SS&CF), simultaneous saccharification and fermentation (SS&F), and modified simultaneous saccharification and fermentation (SS&F) techniques. Drying and wet-milling generated 41.2 g of white starch/100 g flour, and processing with alum gave 58.6/100 g MKS. Hydrolysis of 5 g MKS by sulfuric acid, sodium hydroxide, malted “acha”, and Aspergillus niger amylase for 2 h produced (g/100 mL) 3.97 g, 4.0 g, 4.43 g and 4.24 g of sugar, respectively. Fermentation with 7 g of MKS yielded maximum sugar and ethanol concentrations. Ethanol obtained using SS&CF, SH&F, SS&F and modified SS&F were (v/v); 0.26%, 2.0%, 1.13% and 3.985%, respectively. These results confirmed MKS as a potential feedstock for bioethanol production. [Display omitted] •Mango kernels are abundantly generated waste from mango fruit.•Mango kernel flour was extracted and treated for starch recovery.•Produced starch was used as feed stock for sugar and ethanol production.•Varying fermentation techniques were explored for ethanol production.•Modified simultaneous saccharification & fermentation gave 4.0% ethanol yield. Mango; Mango seed kernel; Mango seed starch; Starch hydrolysis; Sugar fermentation; Bioethanol production.