Molecular cloning of a cDNA encoding a soluble protein in the coral exoskeleton

Organic substances were extracted from the calcified exoskeleton of the reef coral Galaxea fascicularis. In an SDS–PAGE analysis of the extract, a protein with an apparent molecular mass of 53 kDa was detected as well as two other weaker bands. A Ca 2+ overlay analysis failed to find a Ca 2+-binding...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 304; no. 1; pp. 11 - 17
Main Authors Fukuda, Isao, Ooki, Syuuichi, Fujita, Toshihiro, Murayama, Emi, Nagasawa, Hiromichi, Isa, Yeishin, Watanabe, Toshiki
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 25.04.2003
Subjects
Amino Acid Sequence
Anthozoa - anatomy & histology
Anthozoa - metabolism
Anthozoa - physiology
Base Sequence
Calcification
Calcification, Physiologic
Calcium carbonate
Cloning, Molecular
Consensus Sequence
DNA, Complementary
Exoskeleton
Extracellular Matrix Proteins - chemistry
Extracellular Matrix Proteins - genetics
Extracellular Matrix Proteins - metabolism
Galaxea fascicularis
galaxin
Glycoprotein
Glycoproteins - chemistry
Glycoproteins - genetics
Glycoproteins - metabolism
Hermatypic coral
Marine
Molecular Sequence Data
Planula
Repetitive Sequences, Amino Acid
Scleractinia
Exoskeleton
Calcium carbonate
Glycoprotein
Calcification
Planula
Scleractinia
Hermatypic coral
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Summary:Organic substances were extracted from the calcified exoskeleton of the reef coral Galaxea fascicularis. In an SDS–PAGE analysis of the extract, a protein with an apparent molecular mass of 53 kDa was detected as well as two other weaker bands. A Ca 2+ overlay analysis failed to find a Ca 2+-binding protein in the extract. Periodic acid Schiff staining indicated that the 53 kDa protein was glycosylated. A cDNA containing the entire open reading frame for this protein was obtained. Analysis of the deduced protein sequence suggests that the protein, named galaxin, is synthesized as a precursor consisting of a signal peptide, a propeptide sequence, and a mature protein of 298 amino acids. Galaxin exhibits a novel amino acid sequence which is characterized by a tandem repeat structure. Galaxin transcripts were detected in the adult coral, but not in planktonic larvae.
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)00527-8