Notes on the Cultivation of Two Mixotrophic Dinophysis Species and Their Ciliate Prey Mesodinium rubrum
Kleptoplastic mixotrophic species of the genus are cultured by feeding with the ciliate , itself a kleptoplastic mixotroph, that in turn feeds on cryptophytes of the / / (TPG) clade. Optimal culture media for phototrophic growth of and from the Galician Rías (northwest Spain) and culture media and c...
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Published in | Toxins Vol. 10; no. 12; p. 505 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
01.12.2018
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Kleptoplastic mixotrophic species of the genus
are cultured by feeding with the ciliate
, itself a kleptoplastic mixotroph, that in turn feeds on cryptophytes of the
/
/
(TPG) clade. Optimal culture media for phototrophic growth of
and
from the Galician Rías (northwest Spain) and culture media and cryptophyte prey for
from Huelva (southwest Spain) used to feed
, were investigated. Phototrophic growth rates and yields were maximal when
and
were grown in ammonia-containing K(-Si) medium versus f/2(-Si) or L1(-Si) media.
cultures were scaled up to 18 L in a photobioreactor. Large differences in cell toxin quota were observed in the same
strains under different experimental conditions. Yields and duration of exponential growth were maximal for
from Huelva when fed
from the same region, versus
from the Galician Rías or
and
. Limitations for mass cultivation of northern
strains with southern
were overcome using more favorable (1:20)
:
ratios. These subtleties highlight the ciliate strain-specific response to prey and its importance to mass production of
and
cultures. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These co-first authors contributed equally to this work. |
ISSN: | 2072-6651 2072-6651 |
DOI: | 10.3390/toxins10120505 |