Tristetraprolin: A novel target of diallyl disulfide that inhibits the progression of breast cancer

Diallyl disulfide (DADS), a volatile component of garlic oil, has various biological properties, including antioxidant, antiangiogenic and anticancer effects. The present study aimed to explore novel targets of DADS that may slow or stop the progression of breast cancer. First, xenograft tumor model...

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Published inOncology letters Vol. 15; no. 5; pp. 7817 - 7827
Main Authors Xiong, Ting, Liu, Xiao-Wang, Huang, Xue-Long, Xu, Xiong-Feng, Xie, Wei-Quan, Zhang, Su-Jun, Tu, Jian
Format Journal Article
LanguageEnglish
Published Greece Spandidos Publications 01.05.2018
Spandidos Publications UK Ltd
D.A. Spandidos
Subjects
Analysis
Apoptosis
Breast cancer
Cancer therapies
Care and treatment
Cell cycle
Development and progression
Extracellular matrix
Garlic
Health aspects
Immunoglobulins
Inflammation
Laboratory animals
Medical prognosis
Metastasis
Oncology
Proteins
Studies
Tumor necrosis factor-TNF
Tumors
Volatile organic compounds
United States > US
China
breast cancer
tristetraprolin
diallyl disulfide
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Summary:Diallyl disulfide (DADS), a volatile component of garlic oil, has various biological properties, including antioxidant, antiangiogenic and anticancer effects. The present study aimed to explore novel targets of DADS that may slow or stop the progression of breast cancer. First, xenograft tumor models were created by subcutaneously injecting MCF-7 and MDA-MB-231 breast cancer cells into nude mice. Subsequently, western blot analysis was performed to investigate the expression of tristetraprolin (TTP), urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in the xenograft tumors, and cell cultures. Tablet cloning, Transwell and wound healing assays revealed that DADS treatment significantly inhibited the proliferation, invasion and migration of breast cancer cells. In addition, DADS treatment led to significant downregulation of uPA and MMP-9 protein expression, but significantly upregulated TTP expression and . Knocking down TTP expression using small interfering RNA reversed the aforementioned effects of DADS, which suggests TTP is a key target of DADS in inhibiting the progression of breast cancer.
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ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2018.8299