A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a...

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Published inNutrients Vol. 10; no. 10; p. 1356
Main Authors Shigemura, Yasutaka, Iwasaki, Yu, Tateno, Mana, Suzuki, Asahi, Kurokawa, Mihoko, Sato, Yoshio, Sato, Kenji
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 22.09.2018
MDPI AG
Subjects
Adult
Animals
Collagen - administration & dosage
Collagen - blood
Collagen - pharmacology
collagen hydrolysate
collagen peptides
cyclic Pro-Hy
Dipeptides - blood
Eating
Female
fibroblasts
Fibroblasts - drug effects
Gelatin
Humans
Hydroxyproline - blood
Mice
Middle Aged
mouse skin
Peptides - administration & dosage
Peptides - blood
Peptides - pharmacology
Pilot Projects
Pro-Hyp
Protein Hydrolysates - administration & dosage
Protein Hydrolysates - blood
Protein Hydrolysates - pharmacology
Skin
Sus scrofa
Tandem Mass Spectrometry
mouse skin
cyclic Pro-Hy
fibroblasts
Pro-Hyp
collagen peptides
collagen hydrolysate
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Summary:Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Pro-Hyp, in human blood after the ingestion of collagen hydrolysate. The cyclic Pro-Hyp levels in plasma samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma after ingestion of collagen hydrolysate, reaching a maximum level after 2 h and then decreasing. The maximum level of cyclic Pro-Hyp in plasma ranged from 0.1413 to 0.3443 nmol/mL, representing approximately 5% of linear Pro-Hyp in plasma after ingestion of collagen hydrolysate. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp.
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ISSN:2072-6643
2072-6643
DOI:10.3390/nu10101356