Mouse muscle nicotinic acetylcholine receptor γ subunit: cDNA sequence and gene expression

• Published in: Nucleic acids research Vol. 14; no. 8; pp. 3539 - 3555
• Main Authors: Yu, Lei, LaPolla, Robert J., Davidson, Norman
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25.04.1986
• Subjects: acetylcholine; Amino Acid Sequence; Animals; Applied sciences; cDNA; Cells, Cultured; DNA - isolation & purification; DNA - metabolism; DNA Restriction Enzymes; Exact sciences and technology; gene expression; Genes; Humans; Macromolecular Substances; Mice; Mice, Inbred C3H; Nucleic Acid Hybridization; nucleotide sequence; Other techniques and industries; Receptors, Nicotinic - genetics; Species Specificity; Transcription, Genetic
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/14.8.3539

Clones coding for the mouse nicotinic acetylcholine receptor (AChR) γ subunit precursor have been selected from a cDNA library derived from a mouse myogenic cell line and sequenced. The deduced protein sequence consists of a signal peptide of 22 amino acid residues and a mature γ subunit of 497 amino acid residues. There is a high degree of sequence conservation between this mouse sequence and published human and calf AChR γ subunits and, after allowing for functional amino acid substitutions, also to the more distantly related chicken and Torpedo AChR γ subunits. The degree of sequence conservation is especially high in the four putative hydrophobic membrane spanning regions, supporting the assignment of these domains. RNA blot hybridization showed that the mRNA level of the γ subunit increases by 30 fold or more upon differentiation of the two mouse myogenic cell lines, BC3H-1 and C2C12 suggesting that the primary controls for changes in gene expression during differentiation are at the level of transcription. One cDNA clone was found to correspond to a partially processed nuclear transcript containing two as yet unspliced intervening sequences.