Mouse muscle nicotinic acetylcholine receptor γ subunit: cDNA sequence and gene expression
Clones coding for the mouse nicotinic acetylcholine receptor (AChR) γ subunit precursor have been selected from a cDNA library derived from a mouse myogenic cell line and sequenced. The deduced protein sequence consists of a signal peptide of 22 amino acid residues and a mature γ subunit of 497 amin...
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Published in | Nucleic acids research Vol. 14; no. 8; pp. 3539 - 3555 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
25.04.1986
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Subjects | |
Online Access | Get full text |
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Summary: | Clones coding for the mouse nicotinic acetylcholine receptor (AChR) γ subunit precursor have been selected from a cDNA library derived from a mouse myogenic cell line and sequenced. The deduced protein sequence consists of a signal peptide of 22 amino acid residues and a mature γ subunit of 497 amino acid residues. There is a high degree of sequence conservation between this mouse sequence and published human and calf AChR γ subunits and, after allowing for functional amino acid substitutions, also to the more distantly related chicken and Torpedo AChR γ subunits. The degree of sequence conservation is especially high in the four putative hydrophobic membrane spanning regions, supporting the assignment of these domains. RNA blot hybridization showed that the mRNA level of the γ subunit increases by 30 fold or more upon differentiation of the two mouse myogenic cell lines, BC3H-1 and C2C12 suggesting that the primary controls for changes in gene expression during differentiation are at the level of transcription. One cDNA clone was found to correspond to a partially processed nuclear transcript containing two as yet unspliced intervening sequences. |
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Bibliography: | Present address: Johnson & Johnson Biotechnology Center, PO Box 8289, La Jolla, CA 92038, USA ArticleID:14.8.3539 istex:1301DE9B372C71D033587F9254197BD0A1B0C18E ark:/67375/HXZ-V8RTZGQZ-G ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0305-1048 1362-4962 |
DOI: | 10.1093/nar/14.8.3539 |