Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow

• Published in: Immunology letters Vol. 89; no. 2; pp. 267 - 270
• Main Authors: De Ugarte, Daniel A., Alfonso, Zeni, Zuk, Patricia A., Elbarbary, Amir, Zhu, Min, Ashjian, Peter, Benhaim, Prosper, Hedrick, Mare H., Fraser, John K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.10.2003
• Subjects: Adipose; Adipose Tissue - immunology; Antigens, Surface - immunology; Bone Marrow Cells - immunology; Hematopoietic Stem Cells - immunology; Humans; Mesenchymal stem cell; Phenotype; Processed lipoaspirate cell; Mesenchymal stem cell; INF, inteferon; TCR, T-cell receptor; Adipose; NCAm, neural cell adhesion molecule; VCAM, vascular cell adhesion molecule; ALCAM, activated leukocyte adhesion molecule; Phenotype; PGP, phagocytic glycoprotein-1/hyalruonate receptor; CD, cluster of differentiation; LCA, leukocyte common antigen; ATCC, American type culture collection; BD, Becton–Dickinson; ICAM, intercellular adhesion molecule; PECAM, platelet endothelial cell adhesion molecule; Processed lipoaspirate cell; DSHB, developmental studies hybridoma bank; R, receptor
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/S0165-2478(03)00108-1

Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin α4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation.