Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

• Published in: Journal of applied oral science Vol. 26; p. e20170065
• Main Authors: Sordi, Mariane Beatriz, Moreira, Thaís Altoé, Montero, Juan Felipe Dumes, Barbosa, Luis Cláudio, Benfatti, César Augusto Magalhães, Magini, Ricardo de Souza, Pimenta, Andréa de Lima, Souza, Júlio César Matias de
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Faculdade De Odontologia De Bauru - USP 01.01.2018; University of São Paulo
• Subjects: Analysis of Variance; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - pharmacology; Antimicrobial; beta-Lactam Resistance - drug effects; beta-Lactams - chemical synthesis; beta-Lactams - pharmacology; Biofilm; Biofilms - drug effects; Biofilms - growth & development; Colony Count, Microbial; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Dose-Response Relationship, Drug; Gentian Violet; Lactams; Lactones; Lactones - chemical synthesis; Lactones - pharmacology; Microbial Sensitivity Tests; Microbial Viability - drug effects; Microscopy, Electron, Scanning; Original; Plankton - drug effects; Plankton - growth & development; Reproducibility of Results; Streptococcus mutans; Streptococcus mutans - drug effects; Streptococcus mutans - growth & development; Antimicrobial; Biofilm; Lactams; Lactones; Streptococcus mutans
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/1678-7757-2017-0065

Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.