Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel

A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Can...

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Published inJournal of veterinary diagnostic investigation Vol. 13; no. 5; p. 437
Main Authors Kano, R, Sakamoto, Y, Hanahachi, A, Kamata, H, Fukuda, Y, Fujiwara, K, Hasegawa, A
Format Journal Article
LanguageEnglish
Published United States 01.09.2001
Subjects
Animals
Candida - isolation & purification
Candida - pathogenicity
Candidiasis - genetics
Candidiasis - pathology
Candidiasis - veterinary
Crop, Avian - microbiology
Crop, Avian - pathology
Diarrhea - etiology
Diarrhea - veterinary
DNA, Bacterial - analysis
Dyspnea - etiology
Dyspnea - veterinary
Parrots - microbiology
Polymerase Chain Reaction - veterinary
Sequence Analysis, DNA
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Summary:A 2-month-old cockatiel was evaluated for diarrhea, dyspnea, and death. Histologic examination of lesions in the crop mucosa revealed hyperkeratosis and the presence of blastoconidia and hyphae. Positive immunohistochemical staining of the organisms was achieved with an antibody directed against Candida spp. Polymerase chain reaction amplification of DNA from crop lesion material with internal transcribed spacer 2 (ITS2) primers yielded fragments of approximately 300 bp, which demonstrated 95% DNA homology with the corresponding sequence from a strain of Candida parapsilosis deposited in the GenBank data base. The Candida species in the lesion of the crop mucosa was therefore identified by DNA sequence analysis as C. parapsilosis.
ISSN:1040-6387
DOI:10.1177/104063870101300515