Increased Dietary Substrate Delivery Alters Hepatic Fatty Acid Recycling in Healthy Men
Increased Dietary Substrate Delivery Alters Hepatic Fatty Acid Recycling in Healthy Men Maureen T. Timlin , Brian R. Barrows and Elizabeth J. Parks Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota Address correspondence and reprint requests to Elizabeth J. Parks...
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Published in | Diabetes (New York, N.Y.) Vol. 54; no. 9; pp. 2694 - 2701 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Alexandria, VA
American Diabetes Association
01.09.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Increased Dietary Substrate Delivery Alters Hepatic Fatty Acid Recycling in Healthy Men
Maureen T. Timlin ,
Brian R. Barrows and
Elizabeth J. Parks
Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota
Address correspondence and reprint requests to Elizabeth J. Parks, PhD, Department of Food Science and Nutrition, University
of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108. E-mail: eparks{at}umn.edu
Abstract
Sources of fatty acids flowing to the liver may be used for triacylglycerol (TAG) synthesis. Our objective was to quantify
contributions of nonesterified fatty acids (NEFAs), de novo lipogenesis, and dietary fatty acids to VLDL-TAG in the fed state
after meal feeding in healthy subjects ( n = 6). The effect of substrate delivery rate was also determined by comparison with data obtained under a continuous-feeding
regimen. A liquid diet was administered by mouth or via feeding tube. Contributions of NEFAs, de novo lipogenesis, and dietary
fatty acids to VLDL-TAG were quantified using stable isotopes and gas chromatography-mass spectrometry. Contribution of NEFAs
to VLDL-TAG was similar under meal feeding and continuous feeding, although insulin area under the curve (AUC) was greater
under meal feeding (1,597 ± 455 vs. 471 ± 484 pmol · h · l −1 , P < 0.004). Lipogenesis achieved a higher AUC with meal feeding versus continuous feeding (88.7 ± 84.4 vs. 1.9 ± 19.3 μmol
· h · l −1 , P = 0.03) supporting greater stimulation of de novo lipogenesis from increased glucose delivery rate. The contribution of dietary
fatty acids to VLDL-TAG was also greater with meal feeding. These data demonstrate for the first time in humans the well-coordinated
use of fatty acids by the liver during the transition from fasted to fed states and highlight the dominant role of NEFAs for
VLDL-TAG synthesis in both states.
AUC, area under the curve
NEFA, nonesterified fatty acid
TAG, triacylglycerol
Footnotes
Accepted June 1, 2005.
Received September 13, 2004.
DIABETES |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0012-1797 1939-327X |
DOI: | 10.2337/diabetes.54.9.2694 |