Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1

• Published in: Nanomedicine Vol. 9; no. 7; pp. 1018 - 1026
• Main Authors: Lee, Jin-Ho, PhD, Kim, Byung-Chan, PhD, Oh, Byung-Keun, PhD, Choi, Jeong-Woo, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2013
• Subjects: AIDS; Biosensor; Enzyme-Linked Immunosorbent Assay; Genome, Viral - genetics; Gold; HEK293 Cells; HIV-1 - genetics; HIV-1 - immunology; HIV-1 - isolation & purification; HIV-1 virus; Human immunodeficiency virus 1; Humans; Immunoassay; Immunosensor; Internal Medicine; Limit of Detection; Localized surface plasmon resonance; Nanoparticles - ultrastructure; Optical Phenomena; Reproducibility of Results; RNA, Viral - genetics; Staining and Labeling; Surface Plasmon Resonance - instrumentation; Surface Plasmon Resonance - methods; Virion - metabolism; AIDS; HIV-1 virus; Localized surface plasmon resonance; Biosensor; Immunosensor
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2013.03.005

Abstract A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance (LSPR) method. Uniform nanopattern of circular Au-dots (10-20 nm) was fabricated on indium tin oxide (ITO) coated glass substrate by simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the shift of longitudinal wavelength in the UV–Vis spectrum which results from the changes of local refractive index induced by specific antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200 fg/mL, which is 10 fold higher than that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity, it is a promising approach for biological/medical sample analysis and various kinds of virus detection. From the Clinical Editor A localized surface plasmon resonance-based virus detection method is shown to have an order of magnitude improvement in detectable concentration of HIV-1 particles. Similar approaches may be used for screening other viral particles as well.