Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

• Published in: Biomaterials Vol. 23; no. 4; pp. 1205 - 1212
• Main Authors: Park, Si-Nae, Park, Jong-Chul, Kim, Hea Ok, Song, Min Jung, Suh, Hwal
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.02.2002
• Subjects: Animals; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biocompatible Materials - toxicity; Biodegradation, Environmental; Cattle; Cell Line; Collagen; Collagen - chemistry; Collagen - metabolism; Collagen - toxicity; Cross-Linking Reagents; EDC cross-linking; Ethyldimethylaminopropyl Carbodiimide - analogs & derivatives; Hyaluronic acid; Hyaluronic Acid - chemistry; Hyaluronic Acid - metabolism; Hyaluronic Acid - toxicity; Materials Testing; Mice; Microscopy, Electron, Scanning; Porous scaffold; Spectroscopy, Fourier Transform Infrared; Tissue Engineering; EDC cross-linking; Hyaluronic acid; Porous scaffold; Collagen
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(01)00235-6

In order to develop a scaffolding material for tissue regeneration, porous matrices containing collagen and hyaluronic acid were fabricated by freeze drying at –20°C, −70°C or −196°C. The fabricated porous membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1–100 m m concentrations for enhancing mechanical stability of the composite matrix. Scanning electron microscope (SEM) views of the matrices demonstrated that the matrices obtained before cross-linking process had interconnected pores with mean diameters of 40, 90 or 230 μm and porosity of 58–66% according to the freezing temperature, and also the porous structures after cross-linking process were retained. The swelling test and IR spectroscopic measurement of different cross-linked membranes were carried out as a measure of the extent of cross-linking. The swelling behavior of cross-linked membranes showed no significant differences as cross-linking degree increased. FT-IR spectra showed the increase of the intensity of the absorbencies at amide bonds (1655, 1546, 1458 cm −1) compared to that of CH bond (2930 cm −1). In enzymatic degradation test, EDC treated membranes showed significant enhancement of the resistance to collagenase activity in comparison with 0.625% glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity.