Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 80; no. 5; pp. 1421 - 1425
• Main Authors: Moscovici, M. Giovannella, Moscovici, Carlo
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.03.1983; National Acad Sciences
• Subjects: Animals; Avian Leukosis Virus - isolation & purification; avian myeloblastosis virus; Avian Myeloblastosis Virus - genetics; Avian Myeloblastosis Virus - isolation & purification; Avian Myeloblastosis Virus - physiology; Bone marrow cells; Cell Line; Cell lines; Cell Transformation, Viral; Cellular senescence; Chickens; Hematopoietic stem cells; Lesions; Leukemia; Macrophages; Mutation; Phenotype; Temperature; Transformed cell line; Virus Replication; Viruses; Yolk sac
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.80.5.1421

A temperature-sensitive (ts) mutant, GA 907/7, was isolated after mutagen treatment of avian myeloblastosis virus. When bone marrow cells or secondary yolk sac macrophages were infected with GA 907/7, the expression of transformation was greatly reduced at 41 degrees C. The results of temperature-shift experiments suggest that in GA 907/7 the putative v-myb gene product is functional only at 35.5 degrees C. Moreover, when ts-induced transformed cells were shifted to 41 degrees C, a partial morphological conversion to macrophage-like cells was obtained, while the majority of the cells underwent senescence and lysis. No leukemia was obtained when GA 907/7 was injected in 1-day-old chickens. Finally, a continuous cell line releasing genetically stable mutant virus was obtained after transformation of secondary yolk sac cells.