Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene ( tufM) from maize ( Zea mays L.)

• Published in: Gene Vol. 257; no. 2; pp. 233 - 242
• Main Authors: Choi, Kyu Ri, Roh, Kwang-Soo, Kim, Jeong-Kook, Sim, Woong-Soep
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.10.2000
• Subjects: Amino Acid Sequence; Arabidopsis thaliana; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA, Plant - chemistry; DNA, Plant - genetics; DNA, Plant - isolation & purification; elongation factor EF-Tu; Genes, Plant - genetics; Genomic cloning; Intron; Mitochondria - chemistry; Molecular Sequence Data; Peptide Elongation Factor Tu - genetics; Phylogeny; Primer extension; Promoter; Promoter Regions, Genetic; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transcription, Genetic; TufA gene; TufM gene; Zea mays; Zea mays - genetics; Promoter; EF-1α, elongation factor 1α; Intron; EF-Tu, elongation factor Tu; Primer extension; tufA, chloroplast elongation factor TuA gene; Genomic cloning; UTR, untranslated region; HMG, high mobility group; tufB, chloroplast elongation factor TuB gene; tufM, mitochondrial elongation factor Tu gene

We have cloned and characterized a mitochondrial elongation factor Tu (EF-Tu) gene ( tufM) in maize ( Zea mays L.). This maize tufM gene encoded a polypeptide of 452 amino acid residues, consisting of a putative transit peptide of 55 residues and a mature EF-Tu of 397 residues. The coding region was...