Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene ( tufM) from maize ( Zea mays L.)
We have cloned and characterized a mitochondrial elongation factor Tu (EF-Tu) gene ( tufM) in maize ( Zea mays L.). This maize tufM gene encoded a polypeptide of 452 amino acid residues, consisting of a putative transit peptide of 55 residues and a mature EF-Tu of 397 residues. The coding region was...
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Published in | Gene Vol. 257; no. 2; pp. 233 - 242 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
31.10.2000
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Subjects | |
Online Access | Get full text |
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Summary: | We have cloned and characterized a mitochondrial elongation factor Tu (EF-Tu) gene (
tufM) in maize (
Zea mays L.). This maize
tufM gene encoded a polypeptide of 452 amino acid residues, consisting of a putative transit peptide of 55 residues and a mature EF-Tu of 397 residues. The coding region was composed of 12 exons and 11 introns that ranged from 76 to 1673
bp in length. The deduced amino acid sequence showed 85.9% and 61.2% identity with
Arabidopsis mitochondrial EF-Tu and
Arabidopsis chloroplast EF-Tu sequence respectively. The transcription initiation site was determined to be 165
bp upstream of the AUG initiation codon by primer extension analysis. Southern blot analysis revealed that the cloned EF-Tu gene was encoded by the members of small gene family in maize. Although this gene does not resemble the
Arabidopsis nuclear
tufA gene, which encodes the plastid EF-Tu, and does not contain sequence elements found in all cyanobacterial and plastid
tufA genes, the predicted amino acid sequence includes an N-terminal extension that resembles a mitochondrial targeting sequence, and shares three unique sequence elements with mitochondrial EF-Tu's from
Arabidopsis thaliana,
Saccharomyces cerevisiae, and
Homo sapiens. Therefore, we concluded that this gene encodes the maize mitochondrial EF-Tu. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(00)00404-2 |