High expression of PRPS1 induces an anti-apoptotic effect in B-ALL cell lines and predicts an adverse prognosis in Chinese children with B-ALL

• Published in: Oncology letters Vol. 15; no. 4; pp. 4314 - 4322
• Main Authors: Ma, Yimei, An, Xizhou, Guan, Xianmin, Kong, Qinglin, Wang, Yanzhen, Li, Pengfei, Meng, Yan, Cui, Yinghui, Wen, Xianhao, Guo, Yuxia, Shen, Yali, Yu, Jie
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.04.2018; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Acute lymphocytic leukemia; Age; Apoptosis; Biosynthesis; Cancer therapies; Care and treatment; Cell cycle; Chemotherapy; Children & youth; Development and progression; Females; Gene expression; Genetic aspects; Health aspects; Infections; Kinases; Leukemia; Lymphoma; Medical prognosis; Oncology; Pediatrics; Polymerase chain reaction; Proteins; Transcription factors; Transferases; United States > US; China; apoptosis; prognosis; phosphoribosyl pyrophosphate synthetase 1; B-cell lymphoma 2; B-cell acute lymphoblastic leukemia
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2018.7903

Phosphoribosyl pyrophosphate synthetase 1 (PRPS1) is closely associated with a number of diseases; however, its influence in B-cell acute lymphoblastic leukemia (B-ALL) and the potential molecular mechanisms involved remain unclear. The present study aimed to evaluate the expression of PRPS1 in Chinese children with B-ALL and to investigate the mechanism of action of PRPS1 in this disease. A Cell Counting Kit-8 (CCK-8) assay was performed to examine the proliferation of B-ALL Sup-B15 and Raji cells, and flow cytometric analysis was conducted to determine the cell cycle distribution and rate of apoptosis. The mRNA and protein expression levels of PRPS1, MYC proto-oncogene, bHLH transcription factor, cyclin E1, B-cell lymphoma-2 (Bcl-2), cyclin dependent kinase 2 and caspase-3 were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Elevated PRPS1 expression was associated with a high-risk stratification and poor prognosis in patients with B-ALL. Furthermore, overexpression of PRPS1 accelerated the growth of and inhibited apoptosis in Sup-B15 and Raji cells as well as increasing the expression of Bcl-2 to induce an anti-apoptotic effect in B-ALL cell lines. The results of the present study indicate that PRPS1 regulates multiple processes in B-ALL and may be an attractive therapeutic target.