Chemotherapy control by breath profile with application of SPME-GC/MS method

• Published in: Journal of separation science Vol. 35; no. 21; pp. 2908 - 2913
• Main Authors: Ulanowska, Agnieszka, Trawińska, Ewa, Sawrycki, Piotr, Buszewski, Bogusław
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.11.2012; Wiley; Wiley Subscription Services, Inc
• Subjects: Aged; Biological and medical sciences; Breath analysis; Breath Tests - methods; Cancer; Chemotherapy; Chromatography; Drugs; Enrichment; Expired air; Female; Fibers; Gas Chromatography-Mass Spectrometry - methods; General aspects; Humans; Lung cancer; Lung Neoplasms - drug therapy; Lung Neoplasms - metabolism; Lungs; Male; Mass spectrometry; Medical sciences; Metabolites; Middle Aged; Patients; Pharmacology; Solid Phase Microextraction - methods; Solid-phase microextraction (SPME); Tumors; VOCs; Volatile organic compounds; Volatile organic compounds (VOCs); Volatile Organic Compounds - analysis; Volatile Organic Compounds - metabolism; Chemical analysis; Lung cancer; Chemical enrichment; Sample preparation; Bronchus disease; Chemical composition; Trace analysis; Human; Lung disease; Expired air; Healthy subject; Respiratory disease; Coupled method; Patient; Volatile organic compound; Malignant tumor; Solid-phase microextraction (SPME); Solid phase microextraction; Gas chromatography; Chemotherapy; Treatment; Breath analysis; Volatile organic compounds (VOCs); Mass spectrometry; Comparative study; Cancer
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201200333

Chemotherapy used as a treatment against lung cancer has influence on metabolic processes occurring in healthy cells. The changes of biochemical pathways proceeded inside cells might be observed in expired air. In the experiment, breath analysis was carried out before and after anticancer therapy. Expired air samples were collected from 22 patients with a biopsy confirmed lung cancer. Volatile organic compounds present in breath were analyzed by gas chromatography/mass spectrometry. For enrichment of analytes solid‐phase microextraction technique was applied. Eight fibers covered by different sorbents were tested. Carboxen‐polydimethylsiloxane fiber revealed the highest extraction efficiency in relation to analytes in breath. The data showed that cytostatic drugs increase the concentration of acetone and isoprene in the breath collected after chemotherapy. Volatile metabolites of administrated drugs were not identified in expired air.