Biogeochemical activity of microbial biofilms in the water column overlying uranium mine tailings

• Published in: Journal of applied microbiology Vol. 117; no. 4; pp. 1079 - 1094
• Main Authors: Bondici, V.F, Khan, N.H, Swerhone, G.D.W, Dynes, J.J, Lawrence, J.R, Yergeau, E, Wolfaardt, G.M, Warner, J, Korber, D.R
• Format: Journal Article
• Language: English
• Published: Oxford Published for the Society for Applied Bacteriology by Blackwell Science 01.10.2014; Blackwell; Oxford University Press
• Subjects: Actinobacteria; Aquabacterium; bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bioconversions. Hemisynthesis; biofilm; Biofilms; Biofilms - growth & development; Biogeochemistry; Biological and medical sciences; Biotechnology; biotransformation; confocal laser scanning microscopy; Dechloromonas; diversity; ecology; Firmicutes; Fundamental and applied biological sciences. Psychology; high-throughput nucleotide sequencing; hydrocarbons; iron; Methods. Procedures. Technologies; Methylobacterium; microbial communities; microbial growth; microbial phylogenetics; Microbiology; mine tailings; Mining; permeability; Phylogeny; phylotype; polymers; principal component analysis; Soil Pollutants, Radioactive - metabolism; Uranium; Uranium - metabolism; Water Microbiology; water quality; X-radiation; Water; Applied microbiology; Diversity; Ecology; Phylogeny; Biogeochemistry; Uranium; Biotransformation; Mine; Water quality; Biofilm; Microorganism; microbial phylogenetics; biotransformation; diversity; ecology; water quality
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.12593

AIMS: To describe microbial diversity, biofilm composition and biogeochemical potential within biofilms in the water overlying uranium tailings characterized by high pH, high metal concentration and low permeability. METHODS AND RESULTS: To estimate microbial diversity in biofilms formed in water columns overlying uranium mine tailings, culture‐dependent and culture‐independent methods were employed. High‐throughput sequencing revealed the presence of 11 phyla; however, the majority of the sequences were affiliated with four major lineages (Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes) as confirmed by culture‐based methods. Dominant phylotypes were closely related to methylotrophs (Methylobacterium) and bacterial groups able to utilize complex hydrocarbons (Aquabacterium and Dechloromonas). Microbial diversity in biofilms from the 13 m depth was significantly different that in biofilms from 1 to 41 m (P < 0·05). Phylotypes closely related to iron‐reducing bacteria were identified at each depth; whereas sulphate‐, thio‐sulphate‐, sulphite‐ and sulphur‐reducing bacteria, at low abundance, were only detected at lower depths. Confocal scanning laser microscopy (CSLM) was used to investigate polymer quantity and composition of the biofilm components, and principal component analysis of the CLSM data revealed that the relative abundance of α‐L‐fucose and N‐acetyl‐glucosamine/lipopolysaccharide residues separated tailings–water interface biofilms from those from other depths. Reduced (ferrous) iron was detected within all the biofilm samples examined by scanning X‐ray transmission microscopy. CONCLUSIONS: Microbial communities within the water column covering a highly alkaline uranium tailings body form biofilms with microenvironments where iron reduction takes place. SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates the biogeochemical potential of microbial biofilm communities in the water column covering an alkaline uranium tailings body; specifically, the nature of the bacterial groups detected (Aquabacterium, Dechloromonas) and the presence of reduced iron suggest that complex hydrocarbons are available for bacterial growth and geochemical change, such as iron reduction, can occur even though the system bulk phase is predominantly oxic.