Autologous Peripheral Blood Hematopoietic Cell Transplantation in Dogs with T‐Cell Lymphoma

• Published in: Journal of veterinary internal medicine Vol. 28; no. 2; pp. 529 - 537
• Main Authors: Warry, E.E, Willcox, J.L, Suter, S.E
• Format: Journal Article
• Language: English
• Published: United States J.B. Lippincott 01.03.2014; Blackwell Publishing Ltd; John Wiley & Sons, Inc; John Wiley and Sons Inc
• Subjects: adverse effects; Animals; Apheresis; B-lymphocytes; Blood Component Removal - veterinary; Bone marrow; Bone marrow transplantation; CD34; cell transplantation; Chemotherapy; cyclophosphamide; disease diagnosis; Dog Diseases - surgery; Dogs; Engraftment; Female; Hematopoietic Stem Cell Transplantation - methods; Hematopoietic Stem Cell Transplantation - veterinary; irradiation; Lymphoma; Lymphoma, T-Cell - surgery; Lymphoma, T-Cell - veterinary; Male; medicine; Original; remission; separators; Stem cell transplantation; T-lymphocytes; Total body irradiation; Transplantation, Autologous - methods; Transplantation, Autologous - veterinary; Treatment Outcome; Tumors; Whole-Body Irradiation - veterinary; Bone marrow transplantation; Engraftment; Total body irradiation; Apheresis; CD34
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/jvim.12302

BACKGROUND: Peripheral blood hematopoietic cell transplantation (PBHCT) is a feasible treatment option for dogs with B‐cell lymphoma. OBJECTIVE: To examine apheresis and PBHCT outcomes in dogs diagnosed with T‐cell lymphoma (TCL). ANIMALS: Fifteen client‐owned dogs diagnosed with high‐grade TCL. METHODS: After high‐dose cyclophosphamide and rhG‐colony‐stimulating (rhG‐CSF) factor treatment, peripheral blood mononuclear cells were collected using cell separators. The harvested cells then were infused after varying doses of total body irradiation (TBI). Postirradiation adverse effects were managed symptomatically and dogs were discharged upon evidence of hematopoietic engraftment. RESULTS: More than 2 × 10⁶CD34+ cells/kg were harvested from 15/15 dogs. Thirteen of 15 (87%) dogs engrafted appropriately, whereas 2 (13%) of the dogs died in the hospital. One dog developed cutaneous B‐cell lymphoma 120 days post‐PBHCT. The median disease‐free interval and overall survival (OS) of the 13 dogs transplanted in first remission from the time of PBHCT were 184 and 240 days, respectively. Stage and substage of disease at diagnosis had no effect on OS. Two of 13 (15%) dogs were alive 741 and 772 days post‐PBHCT. CONCLUSIONS AND CLINICAL IMPORTANCE: PBHCT may be considered as a treatment option for dogs with TCL.