The Crosslink Formation of 2′-OMe Oligonucleotide Containing 2-Amino-6-vinylpurine Protects mRNA from miRNA-Mediated Silencing

Masking the miRNA binding site: Crosslink‐forming oligonucleotide (CFO) was used for target gene‐specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA–mRNA interactions by recognizing sequences unique to the 3′‐UTR that are inherent in each mRNA.

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Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 14; no. 12; pp. 1427 - 1429
Main Authors Hagihara, Shinya, Lin, Wei-Chen, Kusano, Shuhei, Chao, Xiao-guang, Hori, Tsuneaki, Imoto, Shuhei, Nagatsugi, Fumi
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 19.08.2013
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Animals
Base Sequence
Binding Sites - drug effects
Cross-Linking Reagents - chemistry
Cross-Linking Reagents - pharmacology
crosslinks
Fireflies
gene expression
Gene Silencing - drug effects
HeLa Cells
Humans
Luciferases - chemistry
Luciferases - genetics
MicroRNAs
miRNA
Molecular Sequence Data
mRNA
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - pharmacology
oligonucleotides
Purines
RNA, Messenger - chemistry
RNA, Messenger - metabolism
Vinyl Compounds
miRNA
mRNA
oligonucleotides
crosslinks
gene expression
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Summary:Masking the miRNA binding site: Crosslink‐forming oligonucleotide (CFO) was used for target gene‐specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA–mRNA interactions by recognizing sequences unique to the 3′‐UTR that are inherent in each mRNA.
Bibliography:istex:1FB801069E5B5B5C298F1B6BA11AD6CB8F264654
ArticleID:CBIC201300382
Grant-in-Aid for Scientific Research (B), (S)
Grant-in-Aid for Scientific Research on Innovative Areas
Grant-in-Aid for Young Scientists (B)
ark:/67375/WNG-0LV53VSP-P
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201300382