Identification of existing pharmaceuticals and herbal medicines as inhibitors of SARS-CoV-2 infection

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 118; no. 5; pp. 1 - 8
• Main Authors: Jan, Jia-Tsrong, Cheng, Ting-Jen Rachel, Juang, Yu-Pu, Ma, Hsiu-Hua, Wu, Ying-Ta, Yang, Wen-Bin, Cheng, Cheng-Wei, Chen, Xiaorui, Chou, Ting-Hung, Shie, Jiun-Jie, Cheng, Wei-Chieh, Chein, Rong-Jie, Mao, Shi-Shan, Liang, Pi-Hui, Ma, Che, Hung, Shang-Cheng, Wong, Chi-Huey
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 02.02.2021
• Subjects: Adult; Amino acid sequence; Amino acids; Animals; Antibodies; Antiviral agents; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Antiviral Agents - therapeutic use; Assaying; Biological Sciences; Chlorocebus aethiops; COVID-19; COVID-19 - drug therapy; COVID-19 - epidemiology; COVID-19 - virology; Cricetinae; Disease Models, Animal; DNA-directed RNA polymerase; Drug Repositioning - methods; Female; Hamsters; Herbal medicine; Humans; Male; Mefloquine; Molecular modelling; Mushrooms; Mutation; Nelfinavir; Pandemics; Plant Extracts - pharmacology; Plants; Reagents; RNA polymerase; RNA-directed RNA polymerase; SARS-CoV-2 - drug effects; SARS-CoV-2 - genetics; Severe acute respiratory syndrome coronavirus 2; Spike protein; Vero Cells; Viral diseases; SARS-CoV-2; cell-based and animal studies; drug repurposing; antiviral
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2021579118

The outbreak of COVID-19 caused by SARS-CoV-2 has resulted in more than 50 million confirmed cases and over 1 million deaths worldwide as of November 2020. Currently, there are no effective antivirals approved by the Food and Drug Administration to contain this pandemic except the antiviral agent remdesivir. In addition, the trimeric spike protein on the viral surface is highly glycosylated and almost 200,000 variants with mutations at more than 1,000 positions in its 1,273 amino acid sequence were reported, posing a major challenge in the development of antibodies and vaccines. It is therefore urgently needed to have alternative and timely treatments for the disease. In this study, we used a cell-based infection assay to screen more than 3,000 agents used in humans and animals, including 2,855 small molecules and 190 traditional herbal medicines, and identified 15 active small molecules in concentrations ranging from 0.1 nM to 50 μM. Two enzymatic assays, along with molecular modeling, were then developed to confirm those targeting the virus 3CL protease and the RNA-dependent RNA polymerase. Several water extracts of herbal medicines were active in the cell-based assay and could be further developed as plant-derived anti–SARS-CoV-2 agents. Some of the active compounds identified in the screen were further tested in vivo, and it was found that mefloquine, nelfinavir, and extracts of Ganoderma lucidum (RF3), Perilla frutescens, and Mentha haplocalyx were effective in a challenge study using hamsters as disease model.