Identification and Comparison of Chemosensory Genes in the Antennal Transcriptomes of Eucryptorrhynchus scrobiculatus and E. brandti Fed on Ailanthus altissima
The key to the coexistence of two or more species on the same host is ecological niche separation. Adult and both feed on the tree of heaven, , but on different sections of the plant. Olfaction plays a vital role in foraging for food resources. Chemosensory genes on the antennae, the main organ for...
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Published in | Frontiers in physiology Vol. 9; p. 1652 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
20.11.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The key to the coexistence of two or more species on the same host is ecological niche separation. Adult
and
both feed on the tree of heaven,
, but on different sections of the plant. Olfaction plays a vital role in foraging for food resources. Chemosensory genes on the antennae, the main organ for insect olfaction, might explain their feeding differentiation. In the present study, we identified 130 and 129 putative chemosensory genes in
and
, respectively, by antennal transcriptome sequencing, including 31 odorant-binding proteins (OBPs), 11 chemosensory proteins (CSPs), 49 odorant receptors (ORs), 17 ionotropic receptors (IRs), 19 gustatory receptors (GRs), and three sensory neuron membrane proteins (SNMPs) in
and 28 OBPs, 11 CSPs, 45 ORs, 25 IRs, 17 GRs, and three SNMPs in
. We inferred that
(
),
(
) and
(
),
(
) were putative PBPs by the phylogenetic analysis. We identified species-specific OR transcripts (10 EscrORs and 8 EbraORs) with potential roles in the recognition of specific volatiles of
. In addition to conserved "antennal IRs," we also found several "divergent IRs" orthologues in
and
, such as
,
, and
. Compared with other chemosensory genes, GRs between
and
shared lower amino acid identities, which could explain the different feeding habits of the species. We examined OBP expression patterns in various tissues and sexes. Although amino acid sequence similarities were high between EscrOBPs and EbraOBPs, the homologous OBPs showed different tissue expression pattern between two weevils. Our systematic comparison of chemosensory genes in
and
provides a foundation for studies of olfaction and olfactory differentiation in the two weevils as well as a theoretical basis for studying species differentiation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Invertebrate Physiology, a section of the journal Frontiers in Physiology Edited by: Peng He, Guizhou University, China Reviewed by: Hao Guo, Chinese Academy of Sciences, China; Da-Song Chen, Guangdong Institute of Applied Biological Resources, China; Hetan Chang, Stowers Institute for Medical Research, United States |
ISSN: | 1664-042X 1664-042X |
DOI: | 10.3389/fphys.2018.01652 |