The bphDEF meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphACB genes in Rhodococcus sp. strain RHA1
The bphACB genes responsible for the initial oxidation of the aromatic ring of biphenyl/polychlorinated biphenyls (PCB) to meta-cleavage product in Rhodococcus sp. RHA1 have been characterized. We cloned the 6.1 kb EcoRI fragment containing another extradiol dioxygenase gene ( etbC) which was induce...
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Published in | Gene Vol. 187; no. 1; pp. 141 - 149 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
10.03.1997
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Subjects | |
Online Access | Get full text |
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Summary: | The
bphACB genes responsible for the initial oxidation of the aromatic ring of biphenyl/polychlorinated biphenyls (PCB) to
meta-cleavage product in
Rhodococcus sp. RHA1 have been characterized. We cloned the 6.1 kb
EcoRI fragment containing another extradiol dioxygenase gene (
etbC) which was induced during the growth on ethylbenzene. The
bphD,
bphE and
bphF encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPD) hydrolase, 2-hydroxypenta-2,4-dienoate hydratase and 4-hydroxy-2-oxovalerate aldolase, respectively, were found downstream of
etbC. The deduced amino acid (aa) sequence of RHA1
bphD and
bphE had 27–33% and 32–38% identity, respectively, with those of the corresponding genes in
Pseudomonas. BphE and BphF are closely related to the corresponding homoprotocatechuate
meta-cleavage pathway enzymes of
Escherichia coli C. The
bphD and
bphF were expressed in
E. coli and the BphD activity was detected. The
etbCbphDEF genes were transcribed in biphenyl and ethylbenzene growing cells. Pulsed field gel electrophoresis (PFGE) analysis indicated that RHA1 contains three large linear plasmids. Southern blot analysis indicated that the
meta-cleavage pathway for biphenyl/PCB catabolism in RHA1 is directed by the 390 kb plasmid borne
bphDEF genes located separately from
bphACB gene cluster on the 1100 kb plasmid. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(96)00748-2 |