Sensitive determination of pethidine in body fluids by surface ionization organic mass spectrometry

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 758; no. 1; pp. 117 - 121
• Main Authors: Ishii, Akira, Kurihara, Rina, Watanabe-Suzuki, Kanako, Kumazawa, Takeshi, Seno, Hiroshi, Matsushima, Hajime, Suzuki, Osamu, Katsumata, Yoshinao
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 05.07.2001; Elsevier Science
• Subjects: Analgesics; Analgesics, Opioid - analysis; Analysis; Biological and medical sciences; Body Fluids - chemistry; General pharmacology; Humans; Male; Mass Spectrometry - methods; Medical sciences; Meperidine - analysis; Meperidine - blood; Meperidine - urine; Neuropharmacology; Pethidine; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Pethidine; Drug; Performance evaluation; Human; Urine; Biological fluid; Biochemical analysis; Coupled method; Surface ionization; Oral administration; Opiates; Narcotic analgesic; Blood; Gas chromatography; Clinical biology; Analytical method; Mass spectrometry; Quantitative analysis
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(01)00049-4

We have presented a simple and sensitive method for determining pethidine, a narcotic analgesic drug in body fluids by gas chromatography (GC)/surface ionization organic mass spectrometry (SIOMS). Good linearity was obtained in the range of 0.625–25 ng/ml of whole blood and urine by mass chromatography, and in the range of 0.05–2 ng/ml of whole blood by selected ion monitoring (SIM). Pethidine and diphenylpyraline (internal standard) were extracted from body fluids with Bond Elut Certify cartridges; their recoveries were above 95%. The detection limits (signal-to-noise ratio=3) were estimated to be 0.2 ng/ml of whole blood or urine by mass chromatography, 0.02 ng/ml of whole blood by SIM.