Differential Role for Transcription Factor Oct4 Nucleocytoplasmic Dynamics in Somatic Cell Reprogramming and Self-renewal of Embryonic Stem Cells

• Published in: The Journal of biological chemistry Vol. 288; no. 21; pp. 15085 - 15097
• Main Authors: Oka, Masahiro, Moriyama, Tetsuji, Asally, Munehiro, Kawakami, Koichi, Yoneda, Yoshihiro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.05.2013; American Society for Biochemistry and Molecular Biology
• Subjects: Active Transport, Cell Nucleus - physiology; Animals; Cell Biology; Cell Differentiation - physiology; Cell Nucleus - genetics; Cell Nucleus - metabolism; Differentiation; Embryonic Stem Cell; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Mice; NIH 3T3 Cells; Nuclear Transport; Nucleocytoplasmic Shuttling; Oct4; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Reprogramming; Transcription Factors; Nucleocytoplasmic Shuttling; Oct4; Reprogramming; Transcription Factors; Differentiation; Nuclear Transport; Embryonic Stem Cell
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M112.448837

Oct4 is a member of the POU family of transcription factors and plays a critical role in both maintenance of the undifferentiated state of embryonic stem (ES) cells and in the reprogramming of somatic cells to induced pluripotent stem cells. Oct4 is imported into the nucleus where it functions as a transcription factor; however, the spatiotemporal dynamic behavior of Oct4 remains largely unknown. In the present study we show that Oct4 is a nucleocytoplasmic shuttling protein. Furthermore, although Oct4 mutants with altered nuclear import/export activity were able to maintain the self-renewal of ES cells, they displayed limited potential for cellular reprogramming. These results indicate that the intracellular localization of Oct4, which is dependent on nucleocytoplasmic shuttling, must be more strictly regulated for cellular reprogramming, suggesting that Oct4 plays differential roles in the self-renewal of ES cells and in somatic cell reprogramming. Background: The spatiotemporal dynamic behavior of Oct4 remains largely unknown. Results: Oct4 is a nucleocytoplasmic shuttling protein, and Oct4 mutants with biased nucleocytoplasmic localization show limited potential for cellular reprogramming. Conclusion: An appropriate nuclear retention of Oct4 is critical for cellular reprogramming but not for the self-renewal of ES cells. Significance: Our findings will provide novel insight into the role of Oct4 during cellular reprogramming.