Cordon Bleu serves as a platform at the basal region of microvilli, where it regulates microvillar length through its WH2 domains

Cordon Bleu (Cobl) is a WH2-containing protein believed to act as an actin nucleator. We show that it has a very specific localization in epithelial cells at the basal region of microvilli, a localization unlikely to be involved in actin nucleation. The protein is localized by a central region betwe...

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Published inMolecular biology of the cell Vol. 25; no. 18; pp. 2817 - 2827
Main Authors Wayt, Jessica, Bretscher, Anthony
Format Journal Article
LanguageEnglish
Published United States The American Society for Cell Biology 15.09.2014
Subjects
Animals
Cell Line
HEK293 Cells
Humans
Mice
Microvilli - metabolism
Microvilli - ultrastructure
Protein Structure, Tertiary
Protein Transport
Proteins - chemistry
Proteins - physiology
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Summary:Cordon Bleu (Cobl) is a WH2-containing protein believed to act as an actin nucleator. We show that it has a very specific localization in epithelial cells at the basal region of microvilli, a localization unlikely to be involved in actin nucleation. The protein is localized by a central region between the N-terminal COBL domain and the three C-terminal WH2 domains. Ectopic expression of Cobl shortens apical microvilli, and this requires functional WH2 domains. Proteomic studies reveal that the COBL domain binds several BAR-containing proteins, including SNX9, PACSIN 2/syndapin 2, and ASAP1. ASAP1 is recruited to the base of microvilli by binding the COBL domain through its SH3. We propose that Cobl is localized to the basal region of microvilli both to participate in length regulation and to recruit BAR proteins that associate with the curved membrane found at the microvillar base.
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ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E14-06-1131