Preclinical evaluation of Imatinib does not support its use as an antiviral drug against SARS-CoV-2

• Published in: Antiviral research Vol. 193; p. 105137
• Main Authors: Touret, Franck, Driouich, Jean-Sélim, Cochin, Maxime, Petit, Paul Rémi, Gilles, Magali, Barthélémy, Karine, Moureau, Grégory, Mahon, Francois-Xavier, Malvy, Denis, Solas, Caroline, de Lamballerie, Xavier, Nougairède, Antoine
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2021; Elsevier Masson; The Authors. Published by Elsevier B.V
• Subjects: Animals; Antiviral Agents - pharmacology; Antivirals; Cell Line; Chlorocebus aethiops; Coronavirus; Covid-19; COVID-19 - epidemiology; COVID-19 - virology; COVID-19 Drug Treatment; Drug Evaluation, Preclinical; Drug Repositioning; Enzyme Inhibitors - pharmacology; Epithelium; Female; Humans; Imatinib; Imatinib Mesylate - pharmacology; Life Sciences; Lung - pathology; Male; Mesocricetus; Santé publique et épidémiologie; SARS-CoV-2; SARS-CoV-2 - drug effects; Tyrosine kinase inhibitor; Vero Cells; Virus Replication - drug effects; SARS-CoV-2; Imatinib; Coronavirus; Covid-19; Tyrosine kinase inhibitor; Antivirals
• ISSN: 0166-3542; 1872-9096
• DOI: 10.1016/j.antiviral.2021.105137

Following the emergence of SARS-CoV-2, the search for an effective and rapidly available treatment was initiated worldwide based on repurposing of available drugs. Previous reports described the antiviral activity of certain tyrosine kinase inhibitors (TKIs) targeting the Abelson kinase 2 against pathogenic coronaviruses. Imatinib, one of them, has more than twenty years of safe utilization for the treatment of hematological malignancies. In this context, Imatinib was rapidly evaluated in clinical trials against Covid-19. Here, we present the pre-clinical evaluation of imatinib in multiple models. Our results indicated that imatinib and another TKI, the masitinib, exhibit an antiviral activity in VeroE6 cells. However, imatinib was inactive in a reconstructed bronchial human airway epithelium model. In vivo, imatinib therapy failed to impair SARS-CoV-2 replication in a golden Syrian hamster model despite high concentrations in plasma and in the lung. Overall, these results do not support the use of imatinib and similar TKIs as antivirals in the treatment of Covid-19. •Imatinib and masitinib inhibit SARS-CoV-2 replication in VeroE6.•Imatinib does not block SARS-CoV-2 replication in human bronchial airway epithelia.•Imatinib does not exhibit antiviral activity in a golden Syrian hamster model despite a good lung impregnation.