Effect of reduced glutathione treatment on selenosis, blood selenium concentration and glutathione peroxidase activity after repeated short-term selenium exposure in buffalo calves

• Published in: Toxicology (Amsterdam) Vol. 213; no. 1; pp. 169 - 174
• Main Authors: Deore, Milind Devidas, Srivastava, Anil Kumar, Sharma, Suresh Kumar
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.09.2005; Amsterdam Elsevier Science
• Subjects: Animals; Biological and medical sciences; Buffaloes; Buffaloes - blood; Glutathione; Glutathione - pharmacology; Glutathione peroxidase; Glutathione Peroxidase - blood; Male; Medical sciences; Selenium - blood; Selenium - toxicity; Selenosis; Toxicology; Whole blood selenium concentrations; Glutathione peroxidase; Whole blood selenium concentrations; Selenosis; Buffaloes; Glutathione; Short term; Enzyme; Calf; Ox; Exposure; Biological activity; Whole blood; Vertebrata; Mammalia; Treatment; Peroxidases; Artiodactyla; Oxidoreductases; Selenium; Ungulata
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2005.06.003

Effects of repeated feeding of selenium, when given alone or along with reduced glutathione, on whole blood selenium levels, selenosis and glutathione peroxidase activity, was studied in buffalo calves. After feeding 2.5 mg/kg of BW sodium selenite, good correlation was found between the onset of selenosis and whole blood selenium concentrations. Adverse effects appeared when the whole blood selenium concentrations increased above 2 μg/ml and mortality occurred when they exceeded 3.4 μg/ml. Reduced glutathione, given i.v. at 5 mg/kg of BW arrested the progress of selenosis and prevented mortality which was 100% in the sodium selenite supplemented group; also a reduced whole blood selenium concentration was noted. Whole blood selenium concentrations were a better and more sensitive indicator of selenium status than glutathione peroxidase activity alone.