Photocontrollable mononegaviruses

Mononegaviruses are promising tools as oncolytic vectors and transgene delivery vectors for gene therapy and regenerative medicine. By using the Magnet proteins, which reversibly heterodimerize upon blue light illumination, photocontrollable mononegaviruses (measles and rabies viruses) were generate...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 116; no. 24; pp. 11587 - 11589
Main Authors Tahara, Maino, Takishima, Yuto, Miyamoto, Shohei, Nakatsu, Yuichiro, Someya, Kenji, Sato, Moritoshi, Tani, Kenzaburo, Takeda, Makoto
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 11.06.2019
SeriesBrief Report
Subjects
Animals
Biological Sciences
BRIEF REPORT
Cell Line, Tumor
DNA-Directed RNA Polymerases - genetics
Expression vectors
Gene therapy
Genetic Therapy - methods
Genetic Vectors - genetics
Humans
Illumination
Light
Measles
Measles virus - genetics
Mice, Inbred BALB C
Mice, Nude
Oncolysis
Oncolytic Virotherapy - methods
Oncolytic Viruses - genetics
Proteins
Rabies
Rabies virus - genetics
Regenerative medicine
Transgenes - genetics
Virus Replication - genetics
Viruses
rabies virus
mononegavirus
vector
oncolytic
measles virus
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Summary:Mononegaviruses are promising tools as oncolytic vectors and transgene delivery vectors for gene therapy and regenerative medicine. By using the Magnet proteins, which reversibly heterodimerize upon blue light illumination, photocontrollable mononegaviruses (measles and rabies viruses) were generated. The Magnet proteins were inserted into the flexible domain of viral polymerase, and viruses showed strong replication and oncolytic activities only when the viral polymerases were activated by blue light illumination.
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SourceType-Scholarly Journals-1
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Edited by Diane E. Griffin, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, and approved May 8, 2019 (received for review April 16, 2019)
Author contributions: M. Tahara, M.S., K.T., and M. Takeda designed research; M. Tahara, Y.T., S.M., Y.N., and K.S. performed research; M. Tahara, Y.T., S.M., M.S., K.T., and M. Takeda analyzed data; and M. Tahara and M. Takeda wrote the paper.
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.1906531116