Oxidative stress can affect the gene silencing effect of DOTAP liposome in an in vitro translation system

• Published in: International journal of biological sciences Vol. 7; no. 3; pp. 253 - 260
• Main Authors: Umakoshi, Hiroshi, Tanabe, Tomoyuki, Suga, Keishi, Bui, Huong Thi, Shimanouchi, Toshinori, Kuboi, Ryoichi
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher 01.01.2011
• Subjects: Fatty Acids, Monounsaturated - chemistry; Fatty Acids, Monounsaturated - pharmacology; Gene Silencing - drug effects; Gene Transfer Techniques; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Hydrogen Peroxide - pharmacology; Liposomes - chemistry; Liposomes - metabolism; Membrane Fluidity; Oxidative Stress; Protein Biosynthesis; Quaternary Ammonium Compounds - chemistry; Quaternary Ammonium Compounds - pharmacology; Research Paper; RNA, Messenger - metabolism; gene silencing; Oxidative stress; GFP expression; liposome
• ISSN: 1449-2288; 1449-2288
• DOI: 10.7150/ijbs.7.253

Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its inhibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.