Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ

Purpose: To uncover potential diagnostic biomarkers for endocervical adenocarcinoma (EAC) and adenocarcinoma in situ (AIS). Experimental design: Quantitative label-free liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) peptidomics strategies were employed to profi...

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Published inTechnology in cancer research & treatment Vol. 20; p. 1533033821997825
Main Authors Shi, Feng, Li, Xiaohui, Ma, Zhifang, Luan, Ting, Zhong, Tianying, Gu, Yun
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 2021
Sage Publications Ltd
SAGE Publishing
Subjects
Adenocarcinoma
Biomarkers
Cancer
Ions
Liquid chromatography
Malignancy
Mass spectroscopy
Mucus
Original
Peptides
endocervical adenocarcinoma
label-free
adenocarcinoma in situ
cervical mucus
peptide biomarkers
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Summary:Purpose: To uncover potential diagnostic biomarkers for endocervical adenocarcinoma (EAC) and adenocarcinoma in situ (AIS). Experimental design: Quantitative label-free liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) peptidomics strategies were employed to profile 8 cervical mucus (CM) samples, including 3 EAC cases, 2 AIS cases and 3 normal controls (Ctrl). Results: Among the 3721 exclusive peptides identified, 12 (5 up-regulated and 7 down-regulated) endogenous peptides were significantly expressed in EAC compared to healthy controls (EAC/Ctrl); 10 (7 up-regulated and 3 down-regulated) endogenous peptides were significantly expressed in AIS compared to healthy controls (AIS/Ctrl); 11 (6 up-regulated and 5 down-regulated) endogenous peptides were significantly expressed in EAC compared to AIS (EAC/AIS) (absolute fold change ≥1.5, Benjamini-Hochberg adjusted p-value ≤0.05). Among these identifications, annexin A1 (ANXA1) was found to be down-regulated both in EAC and AIS, and its unique peptide (FIENEEQEYVQTVK) may be promising indicators for cervical glandular epithelial lesions. Conclusion: This is the first study to utilize CM peptidomics in cervical glandular malignancies, which may reveal the novel noninvasive biomarkers for EAC and AIS.
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ISSN:1533-0346
1533-0338
1533-0338
DOI:10.1177/1533033821997825