Development of KASP marker for cytoplasmic male sterility in Nicotiana tabacum and utilization in trait introgression

• Published in: Journal of genetics Vol. 98; no. 3; pp. 1 - 8
• Main Authors: Priya, B. N. V., Reddy, T. Venkata, Chidanand, U., Saiprasad, G. V. S.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.09.2019; Springer; Springer Nature B.V
• Subjects: Adenosine triphosphatase; Alleles; Animal Genetics and Genomics; ATP synthase; Biomedical and Life Sciences; Cytoplasmic male sterility; DNA, Mitochondrial - genetics; Evolutionary Biology; Genetic Markers; Genomes; Genomics; Genotype; Genotyping; Germplasm; Hybrids; Life Sciences; Male sterility; Microbial Genetics and Genomics; Mitochondria; Mitochondrial Proton-Translocating ATPases - genetics; Nicotiana - genetics; Oligonucleotide Array Sequence Analysis; Phenotype; Plant Genetics and Genomics; Plant Infertility - genetics; Pollen - genetics; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Research Article; Seed industry; ATP synthase; Kompetitive allele specific PCR marker; cytoplasmic male sterility; mitochondria; SNP variation
• ISSN: 0022-1333; 0973-7731
• DOI: 10.1007/s12041-019-1128-8

Cytoplasmic male sterility (CMS) is an important trait for large-scale hybrid seed production which avoids manual emasculation and undesired horizontal spread of pollen. Rearrangements in mitochondrial genome in terms of deletions and insertions are frequent causes leading to CMS. Mitochondrial ATP synthase is a multisubunit molecular machine which is involved in synthesis of ATP. In this study, three mutations in ATPase subunit 6 were identified and their cosegregation with male sterility was established using tobacco male sterile hybrids and Nicotiana suvaolensis . A breeder friendly Kompetitive allele specific polymerase chain reaction (KASP) SNP marker was developed for high throughput and quick genotyping. Introgression of this trait into selected germplasm lines ( n = 9 ) was achieved based on foreground for CMS and background selection for recurrent parent using KASP marker and 50K custom tobacco SNP array, respectively. Analysis of genotyping data from SNP array revealed the presence of 88–99% of recurrent parent genome in BC 3 F 1 plants. The selected BC 3 F 1 plants exhibit CMS and are indistinguishable from the fertile recurrent parent (germplasm) in terms of plant morphology.