Sinorhizobium fredii HH103 Invades Lotus burttii by Crack Entry in a Nod Factor-and Surface Polysaccharide-Dependent Manner

• Published in: Molecular plant-microbe interactions Vol. 29; no. 12; pp. 925 - 937
• Main Authors: Acosta-Jurado, Sebastián, Rodríguez-Navarro, Dulce-Nombre, Kawaharada, Yasuyuki, Perea, Juan Fernández, Gil-Serrano, Antonio, Jin, Haojie, An, Qi, Rodríguez-Carvajal, Miguel A, Andersen, Stig U, Sandal, Niels, Stougaard, Jens, Vinardell, José-María, Ruiz-Sainz, José E
• Format: Journal Article
• Language: English
• Published: United States The American Phytopathological Society 01.12.2016
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Host Specificity; Loteae - cytology; Loteae - microbiology; Loteae - physiology; Mutation; Phenotype; Plant Root Nodulation; Plant Roots - cytology; Plant Roots - microbiology; Plant Roots - physiology; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - metabolism; Purines - metabolism; Pyrimidines - metabolism; Sinorhizobium fredii - cytology; Sinorhizobium fredii - genetics; Sinorhizobium fredii - physiology; Symbiosis
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi-09-16-0195-r

Sinorhizobium fredii HH103-Rif , a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rif invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rif . A S. fredii HH103-Rif nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rif mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rif fla mutants nodulated L. burttii. None of the S. fredii HH103-Rif surface-polysaccharide mutants gained effective nodulation with L. japonicus.