Fibrinogen like protein-1 knockdown suppresses the proliferation and metastasis of TU-686 cells and sensitizes laryngeal cancer to LAG-3 blockade

• Published in: Journal of international medical research Vol. 50; no. 9; p. 3000605221126874
• Main Authors: Huang, Jiameng, Huang, Qiang, Xue, Jiyao, Liu, Huiqin, Guo, Yang, Chen, Hui, Zhou, Liang
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.09.2022; Sage Publications Ltd; SAGE Publishing
• Subjects: Cell cycle; Immunotherapy; Laryngeal cancer; Metastasis; Neck; Pre-Clinical Research Report; lymphocyte activating gene 3; invasion; immunotherapy; migration; Fibrinogen like protein-1; laryngeal cancer; epithelial-to-mesenchymal transition
• ISSN: 0300-0605; 1473-2300
• DOI: 10.1177/03000605221126874

Objective To detect the expression of fibrinogen like protein-1 (FGL-1) in laryngeal cancer and evaluate its effect on tumor proliferation, metastasis, and antitumor immunity. Methods ELISA and immunohistochemistry were performed to detect FGL-1 expression in laryngeal cancer. The effects of FGL-1 knockdown on the proliferation, cell cycle progression, apoptosis, migration, and invasion of laryngeal cancer cells were evaluated by the CCK-8, colony formation, flow cytometry, Transwell migration, and western blot assays. We detected changes in tumorigenesis and drug response in vivo following FGL-1 knockdown as well as the effects of anti-LAG3 immunotherapy. Immunohistochemistry was performed to determine CD8 and LAG-3 expression in mouse tumor tissues. Results FGL-1 was highly expressed in the plasma and tumor tissues of laryngeal cancer patients. FGL-1 knockdown suppressed the proliferation of TU-686 cells and inhibited the migration and invasion of laryngeal cancer by blocking epithelial-to-mesenchymal transition. Moreover, silencing FGL-1 inhibited tumorigenicity in vivo and synergized with anti-LAG3 immunotherapy. Conclusions We confirmed the high expression of FGL-1 in laryngeal cancer and identified FGL-1 as a potential marker for immunotherapy in laryngeal cancer.