Exploring cancer metabolism using stable isotope-resolved metabolomics (SIRM)

• Published in: The Journal of biological chemistry Vol. 292; no. 28; pp. 11601 - 11609
• Main Authors: Bruntz, Ronald C., Lane, Andrew N., Higashi, Richard M., Fan, Teresa W.-M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.07.2017; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; cancer; Carbon Isotopes; Cellular Reprogramming - drug effects; Drug Evaluation, Preclinical - methods; Drug Evaluation, Preclinical - trends; Energy Metabolism - drug effects; Fourier Analysis; Humans; isotopic tracer; Magnetic Resonance Spectroscopy; Mass Spectrometry; mass spectrometry (MS); metabolism; metabolomics; Metabolomics - methods; Metabolomics - trends; Minireviews; Neoplasms - drug therapy; Neoplasms - enzymology; Neoplasms - metabolism; Nitrogen Isotopes; nuclear magnetic resonance (NMR); cancer; metabolism; metabolomics; nuclear magnetic resonance (NMR); isotopic tracer; mass spectrometry (MS)
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.R117.776054

Metabolic reprogramming is a hallmark of cancer. The changes in metabolism are adaptive to permit proliferation, survival, and eventually metastasis in a harsh environment. Stable isotope-resolved metabolomics (SIRM) is an approach that uses advanced approaches of NMR and mass spectrometry to analyze the fate of individual atoms from stable isotope-enriched precursors to products to deduce metabolic pathways and networks. The approach can be applied to a wide range of biological systems, including human subjects. This review focuses on the applications of SIRM to cancer metabolism and its use in understanding drug actions.