Detection method for genetically modified potato using an ultra-fast PCR system

• Published in: Food science and biotechnology Vol. 32; no. 9; pp. 1185 - 1191
• Main Authors: Shin, Min Ki, Jeon, Seon Min, Koo, Yong Eui
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.08.2023; Springer Nature B.V; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Food Science; Genetic modification; Genetically engineered organisms; Genetically modified organisms; Insects; Nutrition; Potatoes; Research Article; Vegetables; Viral diseases; 식품과학; GM potato; Event specific qualification; Monitoring; UF-PCR; Solanum tuberosum
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-023-01258-5

Genetically modified (GM) potatoes having resistance to insects and viral diseases, low reducing sugar contents, and black spots for high quality continue to be developed. However, no GM potato has been approved as food or feed in the Republic of Korea as the country adheres to a zero-tolerance policy to unauthorized genetically modified organisms (GMOs). When the self-sufficiency rate is low, a detection method to assess GMOs in crops or other products is necessary. Therefore, a rapid method for two GM potato events (SPS-Y9 and EH92-527-1) using an ultra-fast PCR (UF-PCR) system has been developed, and its specificity, sensitivity, and applicability were demonstrated. UF-PCR can decrease the runtime of PCR by more than half of that needed in conventional methods. However, UF-PCR is not a common method for GMO analysis. This rapid detection method may be useful for GMO analyses in field conditions.