Response of HPRT Gene Fragment Functionalized Gold Nanoparticles to Gamma Ray Irradiation

• Published in: Analytical Sciences Vol. 37; no. 2; pp. 309 - 314
• Main Authors: CHEN, Yu, WANG, Kaikai, CHEN, Feng, CHANG, Shuquan, ZHANG, Haiqian
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10.02.2021; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Assembly; Biological effects; Biomolecules; Chemistry; Covalent bonds; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - metabolism; DNA double-strand break; DNA-AuNPs assembly; Gamma ray detectors; Gamma Rays; Gold; Gold - chemistry; Gold - metabolism; Humans; Hypoxanthine; Hypoxanthine phosphoribosyltransferase; Hypoxanthine Phosphoribosyltransferase - genetics; Hypoxanthine Phosphoribosyltransferase - metabolism; Ionizing radiation; Irradiation; Metal Nanoparticles - chemistry; Nanoparticles; oligonucleotide sequences; Oligonucleotides; Radiation detectors; Radiation effects; Raman spectra; Raman spectroscopy; Spectroscopy; Spectrum analysis; Surface plasmon resonance; Ultraviolet radiation; Ultraviolet spectroscopy; oligonucleotide sequences; Surface plasmon resonance; DNAAuNPs assembly; DNA double-strand break; ionizing radiation; DNA-AuNPs assembly
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.20P248

Radiation-sensitive biomolecules are highly significant for studying biological effects of radiation and developing ionizing radiation detectors based on biomolecules. In this work, we selected hypoxanthine phosphoribosyl transferase gene fragments sensitive to gamma-ray irradiation as a sensing element for radiation detection. The end was modified with thiol groups. The thiol-modified oligonucleotide sequences were coupled to the surface of gold nanoparticles by Au–S covalent bonds. The DNA attached to the surface of gold nanoparticles forms a DNA-AuNPs assembly through base pairing. The assembly was irradiated by gamma rays. And its response to radiation was studied with ultraviolet-visible spectroscopy and surface-enhanced Raman scattering (SERS) spectroscopy techniques. SERS spectroscopy and ultraviolet spectroscopy can detect the response of the DNA-AuNPs assembly to gamma-ray irradiation below 100 and 100 – 250 Gy, respectively. The results indicated that it was feasible to develop a new approach of gamma-ray detectors using biomolecular assemblies of gold nanoparticles.