Assessment of Extramammary Paget Disease by Two-Photon Microscopy
Two-photon microscopy techniques are non-linear optical imaging methods which are gaining momentum in the investigation of fixed tissue sections, fresh tissue or even for experiments. Two-photon excited fluorescence and second harmonic generation are two non-linear optical contrast mechanisms which...
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Published in | Frontiers in medicine Vol. 9; p. 839786 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
25.02.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Two-photon microscopy techniques are non-linear optical imaging methods which are gaining momentum in the investigation of fixed tissue sections, fresh tissue or even for
experiments. Two-photon excited fluorescence and second harmonic generation are two non-linear optical contrast mechanisms which can be simultaneously used for offering complementary information on the tissue architecture. While the former can originate from endogenous autofluorescence sources (e.g., NADH, FAD, elastin, keratin, lipofuscins, or melanin), or exogenous eosin, the latter is generated in fibrillar structures within living organisms (e.g., collagen and myosin). Here we test the ability of both these contrast mechanisms to highlight features of the extramammary Paget disease on fixed tissue sections prepared for standard histological examination using immunohistochemical markers and hematoxylin and eosin staining. We also demonstrate the label-free abilities of both imaging techniques to highlight histological features on unstained fixed tissue sections. The study demonstrated that two-photon microscopy can detect specific cellular features of the extramammary Paget disease in good correlation with histopathological results. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Pathology, a section of the journal Frontiers in Medicine Edited by: Vera Luiza Capelozzi, University of São Paulo, Brazil Reviewed by: Dietrich Schweitzer, Friedrich Schiller University Jena, Germany; Walcy Rosolia Teodoro, University of São Paulo, Brazil; Mirian Sotto, Faculdade de Medicina da Universidade de São Paulo, Brazil These authors have contributed equally to this work and share first authorship |
ISSN: | 2296-858X 2296-858X |
DOI: | 10.3389/fmed.2022.839786 |