In vivo phage display identifies novel peptides for cardiac targeting

Heart failure remains a leading cause of mortality. Therapeutic intervention for heart failure would benefit from targeted delivery to the damaged heart tissue. Here, we applied in vivo peptide phage display coupled with high-throughput Next-Generation Sequencing (NGS) and identified peptides specif...

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Bibliographic Details
Published inScientific reports Vol. 14; no. 1; pp. 12177 - 13
Main Authors Ivanova, Alena, Kohl, Franziska, González-King Garibotti, Hernán, Chalupska, Renata, Cvjetkovic, Aleksander, Firth, Mike, Jennbacken, Karin, Martinsson, Sofia, Silva, Andreia M., Viken, Ida, Wang, Qing-Dong, Wiseman, John, Dekker, Niek
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 28.05.2024
Nature Publishing Group
Nature Portfolio
Subjects
631/1647/48
631/443/592
631/61/2298
Animals
Bioinformatics
Cardiomyocytes
Cell Surface Display Techniques - methods
Clustering
Congestive heart failure
Data analysis
Drug delivery
Drug Delivery Systems
Heart attacks
Heart failure
Heart Failure - metabolism
Heart Failure - therapy
Hep G2 Cells
Hepatocytes
High-Throughput Nucleotide Sequencing
Humanities and Social Sciences
Humans
Identification
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Ischemia
Mice
Mortality
multidisciplinary
Myocytes, Cardiac - metabolism
Next-generation sequencing
Peptide Library
Peptides
Peptides - metabolism
Phage display
Pluripotency
Science
Science (multidisciplinary)
Stem cells
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Summary:Heart failure remains a leading cause of mortality. Therapeutic intervention for heart failure would benefit from targeted delivery to the damaged heart tissue. Here, we applied in vivo peptide phage display coupled with high-throughput Next-Generation Sequencing (NGS) and identified peptides specifically targeting damaged cardiac tissue. We established a bioinformatics pipeline for the identification of cardiac targeting peptides. Hit peptides demonstrated preferential uptake by human induced pluripotent stem cell (iPSC)-derived cardiomyocytes and immortalized mouse HL1 cardiomyocytes, without substantial uptake in human liver HepG2 cells. These novel peptides hold promise for use in targeted drug delivery and regenerative strategies and open new avenues in cardiovascular research and clinical practice.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-62953-9