Isolation of the alkane inducible cytochrome P450 (P450alk) gene from the yeast Candida tropicalis

The gene for the alkane-inducible cytochrome P450, P450alk, has been isolated from the yeast Candida tropicalis by immunoscreening a λgt11 library. Isolation of the gene has been identified on the basis of its inducibility and partial DNA sequence. Transcripts of this gene were induced by alkane to...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 144; no. 1; pp. 251 - 257
Main Authors Sanglard, Dominique, Chen, Chien, Loper, John C.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 14.04.1987
Elsevier
Subjects
Alkanes - pharmacology
Applied sciences
Base Sequence
candida
Candida - enzymology
Candida - genetics
Candida tropicalis
Cloning, Molecular
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
DNA, Fungal - genetics
Enzyme Induction - drug effects
Exact sciences and technology
Genes, Fungal
hidrocarburos
hydrocarbons
hydrocarbure
metalloproteine
metalloproteins
metalproteinas
Other techniques and industries
aa
IPTG
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Summary:The gene for the alkane-inducible cytochrome P450, P450alk, has been isolated from the yeast Candida tropicalis by immunoscreening a λgt11 library. Isolation of the gene has been identified on the basis of its inducibility and partial DNA sequence. Transcripts of this gene were induced by alkane to levels 500 to 1000 fold over those detected in glucose-grown cells. The nucleotide sequence of the 3′ portion of this gene revealed a coding sequence for the heme binding segment characteristic of the P450 gene family.
Bibliography:F60
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(87)80503-X