Upregulation of pigment epithelium-derived factor after laser photocoagulation

• Published in: American journal of ophthalmology Vol. 132; no. 3; pp. 427 - 429
• Main Authors: Ogata, Nahoko, Tombran-Tink, Joyce, Jo, Nobuo, Mrazek, David, Matsumura, Miyo
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.09.2001; Elsevier
• Subjects: Animals; Biological and medical sciences; Cells, Cultured; Diseases of the eye; Eye Proteins; Humans; Laser Coagulation; Medical sciences; Nerve Growth Factors; Pigment Epithelium of Eye - cytology; Pigment Epithelium of Eye - metabolism; Pigment Epithelium of Eye - surgery; Proteins - genetics; Proteins - metabolism; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Retina - metabolism; Retina - surgery; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Serpins - genetics; Serpins - metabolism; Up-Regulation; Cell culture; Rat; Rodentia; Pigment epithelium; Retina; Experimental study; Gene expression; Eye; Photocoagulation; Angiogenesis; Neurotrophic factor; Vertebrata; Mammalia; Surgery; Adult animal; Laser; Epithelial cell; Inhibitor; Biological effect
• ISSN: 0002-9394; 1879-1891
• DOI: 10.1016/S0002-9394(01)01021-2

PURPOSE: To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation. METHODS: Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used. RESULTS: After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation ( P < .005), and they were still higher than before photocoagulation at 2 weeks. CONCLUSION: An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.