Differential Chemokine, Chemokine Receptor and Cytokine Expression in Epstein-Barr Virus-associated Lymphoproliferative Diseases

• Published in: Leukemia & lymphoma Vol. 44; no. 8; pp. 1367 - 1378
• Main Authors: Ohshima, Koichi, Karube, Kennosuke, Hamasaki, Makoto, Tutiya, Takeshi, Yamaguchi, Takahiro, Suefuji, Hiroaki, Suzuki, Keiko, Suzumiya, Junji, Ohga, Shouichi, Kikuchi, Masahiro
• Format: Journal Article
• Language: English
• Published: United States Informa UK Ltd 01.01.2003; Taylor & Francis
• Subjects: Adolescent; Adult; Chemokine; Chemokine R; Chemokines - biosynthesis; Chemokines - genetics; Child; Child, Preschool; Cytokines - biosynthesis; Cytokines - genetics; Ebv; Epstein-Barr virus; Epstein-Barr Virus Infections - complications; Epstein-Barr Virus Infections - immunology; Female; Gene Expression Profiling; Herpesvirus 4, Human; Humans; Immunity, Cellular - genetics; Lymphadenitis - immunology; Lymphadenitis - pathology; Lymphoproliferative Diseases; Lymphoproliferative Disorders - immunology; Lymphoproliferative Disorders - pathology; Lymphoproliferative Disorders - virology; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Receptors, Chemokine - biosynthesis; Receptors, Chemokine - genetics; RNA, Messenger - analysis; T-Lymphocytes - immunology; Th1 Cells - immunology; Th2 Cells - immunology
• ISSN: 1042-8194; 1029-2403
• DOI: 10.1080/1042819031000082984

T cell immunity plays an important role in the clinicopathology of Epstein-Barr virus (EBV)-associated diseases. Acute EBV-induced infectious mononucleosis (IM) is a common self-limiting disease, however, other EBV-associated diseases, including chronic active EBV infection (CAEBV), NK cell lymphoma (NKL), and Hodgkin's lymphoma (HL), exhibit distinct clinical features. Chemokines are members of a family of small-secreted proteins. The relationships between chemokines and the chemokine receptor (R) are thought to be important for selectivity of local immunity. Some chemokines, chemokine R and cytokines closely associate with the T cell subtypes, Th1 and Th2 T cells and cytotoxic cells. To clarify the role of T cell immunity in EBV-associated diseases, we conducted gene expression profiling, using chemokine, chemokine R and cytokine DNA chips. Compared to EBV negative non-specific lymphadenitis, CAEBV and NKL exhibited diffuse down- and up-regulation, respectively, of these gene profiles. IM had a predominantly Th1-type profile, whereas HL had a mixed Th1/Th2-type profile. Reduction of the Th1-type cytokine interferon gamma (IFN-γ) in CAEBV was confirmed by Reverse transcriptase-polymerase chain reaction, whereas IFN-γ expression was markedly enhanced in NKL, and moderately enhanced in IM. Compared to IM, CAEBV showed slight elevation of "regulated upon activation, normal T expressed and secreted" (RANTES), but almost all other genes assayed were down-regulated. NKL exhibited elevated expression of numerous genes, particularly IFN-γ-inducible-10 (IP-10) and monokine induced by IFN-γ (MIG). HL showed variable elevated and reduced expression of various genes, with increased expression of IL-13 receptor and MIG. Our study demonstrated the enormous potential of gene expression profiling for clarifying the pathogenesis of EBV-associated diseases.