Effects of fermented soybean meal on innate immunity‐related gene expressions in nursery pigs acutely challenged with lipopolysaccharides

• Published in: Animal science journal Vol. 86; no. 5; pp. 508 - 516
• Main Authors: Roh, Sang‐Gun, Carroll, Jeffery A, Kim, Sung Woo
• Format: Journal Article
• Language: English
• Published: Australia Japanese Society of Zootechnical Science, Jan. 1999-<Apr. 2003> 01.05.2015; Blackwell Publishing Ltd
• Subjects: adiponectin; Adiponectin - blood; Animal Feed; Animals; antioxidant activity; Antioxidants; blood proteins; blood serum; body weight; catheters; CD40 Ligand - blood; cortisol; diet; Fermentation; fermented soybean meal; Gene Expression; gene expression regulation; genes; glutathione peroxidase; Glutathione Peroxidase - blood; glutathione transferase; Glutathione Transferase - blood; Glycine max; Histocompatibility Antigens Class I - blood; Hogs; Hormones; Hydrocortisone - blood; Immune system; Immunity, Innate - genetics; inflammation; Inflammation - genetics; innate immunity; jugular vein; leukocytes; lipopolysaccharides; Lipopolysaccharides - immunology; Male; messenger RNA; microarray technology; Oligonucleotide Array Sequence Analysis; oligonucleotides; pig; plasma protein; Receptors, Fc - blood; soybean meal; Soybeans; swine; Swine - genetics; Swine - immunology; tumor necrosis factors; Weaning; fermented soybean meal; lipopolysaccharides; plasma protein; pig
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12319

This experiment was to determine if replacing soybean meal with fermented soybean meal (FSBM) would reduce the innate immune response after lipopolysaccharide challenge and the changes of gene expression profiles associated with this response. Forty‐eight 21 day‐old pigs were housed individually and fed three diets for 15 days: CON (a diet without FSBM or spray‐dried plasma protein; SDPP), PP7 (a diet with 7% SDPP), and FS10 (a diet with 10% FSBM). Pigs were fitted with a jugular vein catheters receiving lipopolysaccharide challenge (25 μg/kg body weight (BW)) on day 15. Blood was collected for 5 h at 30‐min intervals to measure cortisol. Expressions of gene transcripts in total RNA from leukocytes were compared using an oligonucleotide microarray at 210 min after lipopolysaccharides injection. Cortisol of FS10 was lower (P < 0.05) than CON after lipopolysaccharides challenge. The expression levels of 17 transcripts, including cytosolic glutathione peroxidase and glutathione S‐transferase A4‐4 were increased (P < 0.05), whereas 23 genes including adiponectin, neonatal Fc receptor and tumor necrosis factor ligand superfamily member 5 were decreased (P < 0.05) in FS10. This study suggests that FSBM‐fed pigs can modulate expression of genes related to inflammatory response and anti‐oxidant activity which can be a potential reason for reduced serum cortisol.