Flavone‐associated resistance of two Lemna species to duckweed weevil attack

• Published in: Ecology and evolution Vol. 12; no. 11; pp. e9459 - n/a
• Main Authors: Lee, Gisuk, Choi, Hanyoung, Joo, Youngsung, Kim, Sang‐Gyu
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2022; John Wiley and Sons Inc; Wiley
• Subjects: Aquatic ecosystems; aquatic plant; Aquatic plants; Bioassays; Chemical Ecology; Defense; Duckweed; duckweed weevil; Eggs; Electrical resistivity; Females; Flavones; Flavonoids; Floating plants; Herbivores; Insects; Jasmonic acid; Larvae; Lemna; Metabolites; Pest resistance; Phytosterols; plant defense; Plant growth; Preferences; Species; Survival; Tanysphyrus lemnae; South Korea; aquatic plant; plant defense; duckweed; duckweed weevil
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.9459

Lemna perpusilla and Lemna minor are free‐floating plants that often live in the same habitat. However, little is known about how they differ in response to herbivore attacks. In this study, we examined the species‐specific resistance of two Lemna species to the duckweed weevil, Tanysphyrus lemnae. The female adults of T. lemnae preferred to lay eggs on L. perpusilla over L. minor. In addition, the larvae of T. lemnae performed better when fed on L. perpusilla than on L. minor. To understand the physiological basis of species‐specific resistance in the two Lemna species, we measured the amounts of jasmonic acid (JA), phytosterols, and flavonoids. Attacks by duckweed weevils increased the levels of JA in the two Lemna species, but these levels did not differ significantly between the two species. Interestingly, the levels of flavones (isoorientin, vitexin, and isovitexin) in L. minor species were higher than those in L. perpusilla. The in vitro bioassay showed that three flavones significantly decreased the survival rate of duckweed weevil larvae. Although L. perpusilla was less resistant to duckweed weevil attack compared to L. minor, L. perpusilla grew faster than L. minor regardless of the duckweed weevil attack. These results suggest that these two Lemna species have different defense strategies against the duckweed weevil. Lemna perpusilla and Lemna minor are free‐floating plants that often live in the same habitat. Two aquatic Lemna species make different growth‐defense tradeoffs against herbivore attack. L. minor accumulates more toxic compounds that function by decreasing the larval survival rates.