LncRNA PITPNA‐AS1 boosts the proliferation and migration of lung squamous cell carcinoma cells by recruiting TAF15 to stabilize HMGB3 mRNA

• Published in: Cancer medicine (Malden, MA) Vol. 9; no. 20; pp. 7706 - 7716
• Main Authors: Ren, Ping, Xing, Lei, Hong, Xiaodong, Chang, Liang, Zhang, Hong
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.10.2020; John Wiley and Sons Inc; Wiley
• Subjects: Antibodies; Antisense RNA; Apoptosis; Apoptosis - genetics; Biomarkers, Tumor; Cancer Biology; Cancer therapies; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cytosol; Flow cytometry; Fluorescent Antibody Technique; Gene expression; Gene Expression Regulation, Neoplastic; HMGB3; HMGB3 Protein - genetics; HMGB3 Protein - metabolism; Humans; Investigations; Liver cancer; Lung cancer; Lung carcinoma; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; lung squamous cell carcinoma (LUSC); Metastasis; Microscopy; mRNA stability; Original Research; PITPNA antisense RNA 1 (PITPA‐AS1); Plasmids; Proteins; Reagents; RNA Stability; RNA, Long Noncoding - genetics; Squamous cell carcinoma; TAF15; TATA-Binding Protein Associated Factors - genetics; TATA-Binding Protein Associated Factors - metabolism; China; Japan; HMGB3; TAF15; PITPNA antisense RNA 1 (PITPA-AS1); lung squamous cell carcinoma (LUSC)
• ISSN: 2045-7634; 2045-7634
• DOI: 10.1002/cam4.3268

Plenty of reports have probed the involvement of abnormally expressed lncRNAs in multiple cancers, including lung squamous cell carcinoma (LUSC). Through online database GEPIA, lncRNA PITPNA antisense RNA 1 (PITPNA‐AS1) was highly expressed in LUSC samples, and these tendency was further affirmed in LUSC cells. The aim of current study was to investigate the related mechanism of PITPNA‐AS1 in LUSC. Functional experiments verified that depletion of PITPNA‐AS1 hampered the proliferative and migratory abilities, but accelerated apoptosis of LUSC cells. Additionally, we observed the increased expression of HMGB3 and its positive correlation with PITPNA‐AS1 in LUSC samples. Interestingly, PITPNA‐AS1 mainly located in the cytosol of LUSC cells, and also affected mRNA stability of HMGB3. Furthermore, the repressed mRNA stability of HMGB3 by PITPNA‐AS1 via TAF15 was exposed through mechanism experiments. The mediatory function of PITPNA‐AS1 on HMGB3 was validated via rescue assays. All in all, PITPNA‐AS1 promoted the proliferation and migration of LUSC cells via stabilizing HMGB3 by TAF15. In conclusion, our study displayed a novel mechanism underlying PITPNA‐AS1 in LUSC cells. PITPNA‐AS1 is dramatically elevated and promotive in LUSC cells. PITPNA‐AS1 positively affects HMGB3 in LUSC cells. PITPNA‐AS1 and TAF15 mediates HMGB3 synergistically.