Integrating high‐throughput screening and sequencing for monoclonal antibody discovery and engineering

• Published in: Immunology Vol. 153; no. 1; pp. 31 - 41
• Main Authors: Parola, Cristina, Neumeier, Daniel, Reddy, Sai T.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.01.2018; John Wiley and Sons Inc
• Subjects: Animals; antibodies; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; bioinformatics; Drug Discovery - methods; Engineering; Genetic Engineering - methods; High-Throughput Nucleotide Sequencing - methods; High-throughput screening; High-Throughput Screening Assays - methods; high‐throughput sequencing; Humans; Monoclonal antibodies; Next-generation sequencing; Review; Screening; surface display; surface display; antibodies; high-throughput sequencing; bioinformatics
• ISSN: 0019-2805; 1365-2567
• DOI: 10.1111/imm.12838

Summary Monoclonal antibody discovery and engineering is a field that has traditionally been dominated by high‐throughput screening platforms (e.g. hybridomas and surface display). In recent years the emergence of high‐throughput sequencing has made it possible to obtain large‐scale information on antibody repertoire diversity. Additionally, it has now become more routine to perform high‐throughput sequencing on antibody repertoires to also directly discover antibodies. In this review, we provide an overview of the progress in this field to date and show how high‐throughput screening and sequencing are converging to deliver powerful new workflows for monoclonal antibody discovery and engineering. In this review, we provide an overview of the progress on how high‐throughput screening and sequencing are converging to deliver powerful new workflows for monoclonal antibody discovery and engineering.